Caprine husbandry practices at the prehistoric site of Monjukli Depe in southern Turkmenistan: A Multi-Isotope Perspective

Jana Eger (

Department of History and Cultural Studies at the Free University of Berlin

Sheep and goat predominate in the Neolithic and Aneolithic/Chalcolithic faunal assemblages in southern Central Asia and adjacent microregions, yet their husbandry is still incompletely understood. Some aspects of caprine herding practices, such as the annual timing/seasons of birth and feeding in the context of local conditions, have received little attention so far. This case study addresses these aspects by using an integrated approach combining a multi-isotope analysis (nitrogen, carbon, oxygen, and strontium) mainly on caprine teeth/bones with zooarchaeological results and archaeobotanical data from Monjukli Depe in order to explore the life histories of animals herded at the site. Monjukli Depe is in southern Turkmenistan and inhabited in the late Neolithic (ca. 6200-5650 BCE) and again in the early Aeneolithic (ca. 4800-4350 BCE). Excavations at this site have yielded a large, well-preserved animal bone assemblage.

Passing herd along the Kopet Dag behind the yurt during excavation. Photo: Monjukli Depe Project.

In the first step of the research, bone collagen was extracted from mandibles of sheep and goat and analysed for carbon and nitrogen isotopes (FIG 1). A total of 23 caprine individuals were sampled in the study. From this we can detect past environmental conditions and the range of vegetation to which the animals had continuous access during their lives, including their reliance on C3 and C4 plants. While both plant groups occur in southern Turkmenistan today, the preliminary archaeobotanical data from Monjukli Depe point to a predominance of C3 plants.

Jana sampling material for stable isotope analysis at the chemical lab of the Curt-Engelhorn–Center for Archaeometry GmbH, Mannheim (Germany). Credit: Martin Riesenberg.

The next step, was to sequentially sample lower 3rd molars from the sampled mandibles and analysed for carbon and oxygen isotopes. The δ18O and δ13C values provide insights into seasonal changes in diet and fodder composition over the annual cycle. Several second molars were sampled in addition, so that ca. 11 to 12 months of the first year of the animals’ lives were included in the analyses.

δ18O (white circles) and δ13C (black circles) values, and 87Sr/86Sr ratios (black dashes) for two sheep individuals of Monjukli Depe with both second and third molar sampled (Credit: Jana Eger)

In a third step, the δ18O sequences obtained from the teeth were used to assess strontium isotope analysis. From each tooth two sample positions of the highest and lowest δ18O values were selected to measure the strontium ratios (87Sr/86Sr) to investigate the possibility of different grazing locations on a seasonal scale. The δ18O sequences were further used to provide information about the birth seasonality in the Monjukli herd population.

The analysis reveals that a large proportion of caprine individuals received a more diverse vegetation than others with possible contribution of C4 plants in diet – both on a seasonal and long-term basis. Furthermore, the results support a scenario in which sheep and goats were kept in small flocks at ecologically different locations close to the settlement. Herding practices in the Monjukli case seemed to focus on landscape micro-variability rather than exploiting advantages of altitude differences. The study further indicates that sheep and goat were born across multiple seasons within the annual cycle, and they were raised to maintain a continuous supply of fresh milk along with tender meat, consistent with previous suggestions that husbandry was both meat- and milk-oriented.

Overall, the information gained from the investigation helps to draw a more detailed picture of sheep and goat husbandry, including a better understanding of breeding practices and controlled herd security by the people who tended the animals. The data provide evidence of a wide zootechnical knowledge in this early village society.

The results of the multi-isotope investigation, recently published and online accessible here:, were viewed through the lens of the interdisciplinary field of Human-Animal Studies, which direct their focus to complex and multidimensional interspecies relations . The interpretation included zooarchaeological study of over 50,000 animal remains and the analyses of zoomorphic representations in the form of miniaturized clay objects. Thus, the stable isotopic results are considered within the wider socio-cultural practices and conceptions of relations between humans and (other) animals at the settlement of Monjukli Depe.

This research derives from the Monjukli Depe Project at the Free University of Berlin (FUB) under the direction of Prof. Dr. Susan Pollock and Prof. Dr. Reinhard Bernbeck, and was undertaken as part of my PhD (2014-2020) within the frame of the Berlin Graduate School of Ancient Studies. The financial support for this study was provided by the German Research Foundation, and Frauenfördermittel of the Department of History and Cultural Studies at FUB.

Online Occasional Seminar Series: A place to showcase YOUR research

Beginning in May, with Thiago Hermenegildo and the diet of ducks in pre-Columbian sites of Amazonia, the SIWG has started a new initiative. Researchers are invite to give a short presentation of their research to the community. The session is planned to be a maximum of 1hr.

The next session will be on June 15th 3pm where Jana Eger will present her study of early Aeneolithic animal husbandry at Monjukli Depe, southern Turkmenistan.

Interested? Register here

A brief hiatus……

My apologies dear SIWG members and followers of this blog for the brief hiatus in blog posts. As you can imagine with the opening of borders with the advent of the COVID vaccines, there has been a race to catch up fieldwork and sample collections. As a result, the contributions from researchers have slowed to a halt.

In addition, I will be moving from ICArEHB in Portugal (☹) to a new position in Berlin at the DAI (😊) as their new archaeozoologist. This has meant that I have been focused on finishing academic and personal projects before my family and I move to Berlin in December. I am very sad to be leaving Portugal, but we have built an amazing new isotope preparation lab and I will continue to be providing support to the ICArEHB family in stable isotopes and archaeozoology. But I am also very excited to start at the DAI, and looking forward to building new projects with colleagues in Germany and across the world.

To kick start the new ‘term/semester’, I have been thinking of blog posts about open access databases (IsoArch, ISOBANK and Neotoma), following on from Suzanna Pilaar-Birch’s workshop with the Neotoma database, and discussing the merits of each and which one you should choose; to ensuring good standards of reporting methods and results for transparency. However, I thought I would start the to start with a discussion about landscape reconstruction.

Last week, I published together with my colleagues from Kiel and Belgrade, a paper about the livestock management and land-use during the late Vinča period in Serbia at the incredible sites of Vinča-Belo brdo and Stubline. This study is a part of a large sampling program of bones and teeth from domesticate and wild animals from the two sites. Our first paper concerned the stable isotope results from bone collagen. In this paper, we found a large variability in δ13C/δ15N results in cattle from Vinča-Belo brdo in comparison form cattle from Stubline, who may have been pastured in forested/wetland areas. While sheep and pigs appeared to have been fed similar diets possibly being kept within the settlements. Wild animals, mainly deer and wild pigs, ranged wide habits including forests (figure of results).

The results from stable carbon and nitrogen isotopic analysis of bone collagen samples from Vinča-Belo brdo.
The results from stable carbon and nitrogen isotopic analysis of bone collagen samples from Stubline.

The recently published paper in PLOS-ONE focused on the results from the sequential sampling of bioapatite from cattle, sheep/goat, pig, and deer teeth. Sequential sampling allows an examination of individual life histories, via δ18O/δ13C. The oxygen isotope provides a seasonal framework to examine variation in plant δ13C foliar values. Carbon isotopes can detect different plant types e.g. C3 plants which a predominant plant type found in Serbia. The carbon isotopes of C3 plants are particularly sensitive to changes in their growing conditions 1. Our results echoed the results from the bone collagen analysis but also provided more clarity about the types of pasture that different species may have used and highlighted potential differences in management practices for sheep and cattle.

The variation seen in cattle bone collagen prevailed in the bioapatite samples, with little similarity between individuals (see here). Some individuals were grazed with open environments while others may have been kept for parts of the year in forested/waterlogged environments. Whereas sheep appeared to be managed very similar at both sites with little variation between individuals (see here). What is intriguing is the variation in δ13C from   ̶ 8‰ to   ̶ 14‰, with the depleted values occurring during winter.

We proposed that this variation was in part by plants growing under high temperatures during summer, perhaps within halophyte communities within relic river channels, when one considers the dynamic fluvial landscape of the Danube. During winter, herds may have been kept within the settlements and fed on collected fodder, such as leafy hay from riverine forest environments. Overall, it supports a uniform herding strategy in contrast with that of cattle.

Pasture reconstruction based on study results. The base images were made available on the ‘Nature scenes with fields’ licensed to REG. The animal silhouettes are available under CC 4.0 from

This is one interpretation of the results, and another group of researchers may interpret the data differently, for example Balasse et al. 20172. Balasse et al. (including myself) studied the material from Popină Borduşani along with other Late Neolithic/Eneolithic sites from the Danubian corridor in Romania. Again, bone collagen and enamel bioapatite wild and domesticated animals were analysed providing a multi-faceted view of animal management. Similar enriched carbon isotope values were interpreted as potential the consumption of C4 plants growing as weeds near the settlement.

These studies highlight differences in pasture use and management that may occur in similar environments. To increase our understanding of past herding practices, future studies need carry out comprehensive sampling and analytical programs of wild and domesticated animals from well preserved archaeological sites, where both bone collagen and enamel bioapatite ared analysed. This would allow for greater resolution of past husbandry practices as well as the landscape in which these herds lived and died.

To move forward to create better interpretive frameworks, we need to build detailed databases of carbon and nitrogen, as well as other isotopes, from ecological studies and analysis of local reference material much in the way is crucial for Strontium isotopes studies. We may never be able to say definitively sheep X lived off a diet of turnips in the winter and rich clover meadows during summer. But we can move to increasing the resolution of past herding strategies via improved sampling and multi-isotopic approaches.

1             Tieszen, L. T. Natural variations in the carbon isotope values of plants: Implications for Archaeology, Ecology and Paleoecology. Journal of Archaeological Science 18, 227-248 (1991).

2             Balasse, M. et al. Investigating the scale of herding in Chalcolithic pastoral communities settled along the Danube River in the 5th millennium BC: A case study at Borduşani-Popină and Hârşova-tell (Romania). Quat. Int. 436, 29-40, doi:10.1016/j.quaint.2015.07.030 (2017).

Gray wolves survived Ice Age extinction event by changing their diets – which may help them cope with ongoing climate change

Zoe Landry @zoey_landry

Master’s student in Biology at Carleton University, soon to be PhD student at the University of Ottawa and member of the SAiVE Lab

Climate change is arguably one of the biggest issues that the Earth is currently facing. Ecosystems all over the world are being affected in different ways, with northern ecosystems being some of the most severely impacted. The Canadian Arctic is currently warming at nearly double the rate of the global average, which is drastically altering the Arctic environment. The effects of climate change will be felt long past our lifetimes, but how exactly climate change will impact northern species, some of which are not found anywhere else in the world, is unknown.

            An unparalleled tool for investigating the long-term responses of species to climate change is, fortunately, available in the form of the fossil record. In the Yukon Territory of Canada, there is a diverse array of fossils from the last Ice Age that provide an excellent opportunity to study the effects of climate change on ancient animals. The end of the Ice Age is characterized by a global shift in climate, with warming temperatures and melting glaciers, culminating in an extinction event where about 70% of large mammal genera died out in North America – similar to what we are seeing in the Canadian Arctic today. One of the most abundant fossil animals found in the Yukon is the gray wolf (Canis lupus), which to this day still calls the Yukon home. Ancient wolves managed to survive the extinction event at the end of the Ice Age, but how did they do so, when so many other species did not? Could wolves have changed aspects of their ecology, such as their diets, over time to cope with environmental changes?

Zoe Landry, lead author of the study, holds a 40,000-year-old gray wolf skull from the Yukon. Credit: Danielle Fraser, © Canadian Museum of Nature.

My colleagues and I set out to investigate these questions, using a combination of two different methods: dental microwear and stable isotope analyses in a recent paper. We studied both Ice Age and recent (circa 1960s) gray wolf specimens from the Canadian Museum of Nature, in Ottawa, Ontario, and the Beringia Interpretive Center (Yukon Government), in Whitehorse, Yukon. By comparing the dietary ecology of modern and ancient wolves, we can better understand how wolves responded to past climatic disturbances, and how they may respond at present and in the future.

Zoe Landry and Danielle Fraser prepare for sampling.

Dental microwear analysis

            Dental microwear analysis involves observing microscopic wear patterns on the surface of animal teeth. Through observations of these patterns, we can make inferences about the types of food an animal ate during the last few days to weeks prior to its life. There are two main types of dental wear: pits and scratches. Pits are small, circular features that are caused by an animal consuming hard food – in the case of carnivores, pits indicate that the animal was eating bones. Scratches are long, linear features that are generated when the teeth of carnivores slide against each other to slice meat from prey.

Our dental microwear analysis revealed very similar wear patterns between both the Ice Age wolves and the recent wolves, which indicated that both groups primarily consumed flesh. This is especially interesting, as previous studies had suggested that during the Ice Age, wolves were scavengers that fed on the remains of animals that other carnivores had killed. We can conclude that gray wolves have maintained their status as competitive hunters over thousands of years, and that actively taking down prey is an important aspect of wolf ecology that is unlikely to change even as their habitat does.

Top: Taking moulds of teeth; Bottom: SEM of tooth surface. Credit: Danielle Fraser

Stable isotope analysis

Stable isotope analyses were performed at the University of California, Merced, by Dr. Sora Kim and Dr. Robin B. Trayler. We analyzed the δ13C and δ15N values from both bone carbonates and collagen. There were minimal changes in the δ13C values between the two groups, which suggest that wolves have remained generalist predators that hunted a variety of large herbivores. There was a very clear difference in δ15N values between the Ice Age and recent wolves, which we determined to be driven by a change in the main prey species that the wolves were hunting.

Zoe sampling material for stable isotope analysis. Credit: Danielle Fraser

Based on diet modelling, we discovered that the primary prey of wolves during the Ice Age were horses (Equus sp.), with moderate contributions from other herbivores, such as caribou (Rangifer tarandus) and muskox (Ovibos moschatus). Obviously, there are not huge herds of wild horses in the Yukon today, as they were one of the species that went extinct in North America at the end of the Ice Age. Instead, gray wolves that inhabit the modern Yukon rely mainly on hunting caribou and moose (Alces alces) to fulfill their dietary needs. This shift from a diet composed largely of horse to one of caribou and moose indicate that the survival of wolves is not fixed on a single prey species. Rather, gray wolves are able to shift their diets from one species to another, and are capable of surviving significant climatic and environmental changes, provided that they have access to a sufficiently large population of large-bodied herbivores.

Implications for wolf conservation

            Our study revealed that gray wolves from the Yukon were able to survive the threat of extinction at the end of the Ice Age by changing their diets to target different prey species, and are very adaptable predators. The key to modern wolf survival is the survival of their current prey species, caribou and moose. Conservation efforts should focus on preserving these large herbivores, and ensuring that their populations remain healthy and at stable sizes as the Arctic environment changes. The continued survival of grey wolves in the Yukon is directly linked to these animals, which further demonstrates the important role of caribou and moose in the Canadian Arctic.

We acknowledge that the institutions at which this research took place are located on the traditional and unceded territories of the Algonquin, Anishnaabeg People, the Yokuts and Miwuk People, the Taa’an Kwächän and Kwanlin Dün People. We are grateful to the placer gold mining community and the Tr’ond¨ek Hw¨ech’in First Nation for their continued support and partnership with our research in the Klondike goldfields region; and the Vuntut Gwitchin First Nation for their collaboration with research in the Old Crow region. This research was funded by: Canadian Museum of Nature Research Activity Grant, and Natural Sciences and Engineering Research Council of Canada Discovery Grant (RGPIN-2018-05305), awarded to my supervisor, Dr. Danielle Fraser; and by funds of the Yukon Palaeontology Program of the Yukon Department of Tourism and Culture, contributed by Dr. Grant Zazula.

Seaweed-eating sheep and crop fertilisation trials on the Orkney Islands, Scotland

Magdalena Blanz, Vienna Institute for Archaeological Science (VIAS)

Seaweed consumption

In modern Europe, for most the idea of eating seaweed may seem strange. Outside of sushi, and memories of beach holidays where rotting algae spoiled the fun, most people today have no particular connection to seaweed. It may therefore come as a surprise that a fellow mammal, namely sheep (Ovis aries), can subsist nearly exclusively on seaweed. The most well-known breed of seaweed-eating sheep today are located on North Ronaldsay, the northernmost island of the Scottish Orkney archipelago. Elevated sheep δ13C values attributable to seaweed consumption were common in Neolithic Orkney as well, as the last two decades of archaeological research have shown (cf. Balasse et al. 2019, 2005 for a review).

Seaweed-eating sheep on North Ronaldsay. Picture by Jasmijn Sybenga.

Since the interpretation of archaeological data relies on modern comparative data to gain an understanding of what individual values mean in the archaeological context, analyses of modern material are required. Our study, recently published in the Journal of Archaeological Science, was conceived to collect further modern baseline δ13C and δ15N data for seaweed, terrestrial vegetation, and sheep bone collagen on the Orkney Islands. The results showed extremes of −10.7‰ for δ13C and +10.4‰ δ15N for modern seaweed-eating sheep bone collagen, and a δ13C average of −17.4 ± 1.4‰ for the analysed seaweeds (n = 20; mostly kelps). This modern dataset will help with future identification of seaweed-consumption, and allows an improved assessment in archaeological contexts of how much seaweed was likely consumed when the analysed bone formed.

Seaweed fertilisation

In addition to the consumption of seaweed, the use of seaweed as a fertiliser for terrestrial crops has also been suggested to influence consumer δ13C and δ15N values. To test this, we performed a field trial on Orkney where we fertilised bere barley (a six-row, hulled barley landrace) with seaweed. We followed traditional, historically attested practices with respect to the time and amount of fertilisation, but used a tractor for power-harrowing, sowing and rolling to reduce the still considerable amount of manual labour.

Fertilisation of 3 m × 3 m plots with seaweed (top left) and growth and harvest of the seaweed-fertilised bere barley. Pictures by Peter Martin, John Wishart and Magdalena Blanz.

Isotope ratio analysis showed significant elevation in δ15N values for seaweed-fertilised barley compared to barley from unfertilised control plots, but no significant difference in δ13C. This can be explained by the differing sources of carbon and nitrogen: Since barley takes up nitrogen from the soil (as nitrate, NO3, and ammonium, NH4+), the change in δ15N values upon fertilisation is due to the elevated δ15N values in the seaweed compared to the bioaccessible nitrogen in the unfertilised soil. In contrast, the lack of any major effects of seaweed fertilisation on δ13C is due to barley taking up most carbon by photosynthesis from the air (as carbon dioxide, CO2): Carbon stemming from the fertilisation at the roots has little likelihood of being taken up by the plant.

These results show that fertilisation of terrestrial crops with seaweed does not lead to these crops having more “marine” δ13C values, but that, similar to fertilisation with animal dung, δ15N values are affected by seaweed fertilisation. Further detail on the seaweed fertilisation field trial is available in Blanz et al., 2019 and Brown et al., 2020.

This research was undertaken as part of my PhD (2016-2020) at the Archaeology Institute, Orkney, supervised by Ingrid Mainland, Philippa Ascough, Mark Taggart and Jörg Feldmann. It was funded by the European Social Fund and Scottish Funding Council as part of Developing Scotland’s Workforce in the Scotland 2014–2020 European Structural and Investment Fund Programme.

Lab Experience during a Pandemic

Adam Andrews, Department of Biological, Geological, and Environmental Sciences, University of Bologna, Italy

Rachel Winter, Groningen Institute of Archaeology, University of Groningen, Netherlands

Willemien de Kock, Groningen Institute of Archaeology and Groningen Institute of Evolutionary Life Sciences, University of Groningen, Netherlands

SeaChanges project

SeaChanges (2019-2023) is a MSCA-multidisciplinary international training network of 15 PhD projects exploring long-term perspectives on the exploitation of marine vertebrates by integrating archaeology and marine biology. As a part of the training provided by the network, students undertake research secondments. Here we provide an account of our experiences at the University of York (UK) to complete collagen extraction and stable isotope analysis to contribute to our projects. 

To briefly acquaint you with our projects, small summaries are provided below.

ESR 12 – Adam Andrews, University of Bologna, Italy. I am investigating adaptation in Atlantic bluefin tuna (Thunnus thynnus) throughout the last 6000 years in the Mediterranean and Black Sea. I combine genomics, isotopes, and morphological analyses to understand how adaptable this species was/is and how resilient it remains now which may allow us to predict how it may respond to future change. 

ESR 13 – Rachel Winter, I am based at the University of Groningen in the Netherlands and am combining traditional zooarchaeology (NISP, osteometrics) with biomolecular archaeology (proteomics, stable isotope analysis) and marine ecology to understand the exploitation of groupers (Epinephelus spp.) in the Levant from the Bronze Age through the Hellenistic period. 

ESR 14 – Willemien de Kock, also based at the University of Groningen I study the foraging ecology and genetic connectivity of ancient green turtles (Chelonia mydas) along the Levant. Sea turtle bones are found at various archaeological sites, but my research focuses on remains from Kinet Höyük (Turkey), Tell Fadous-Kfarabida, and Tell el-Burak (both in Lebanon).

Lab Experience during COVID

Conducting lab work during a pandemic is certainly a peculiar experience.

It was a trip that was stressful before it began; for the Groningen delegation it meant traveling despite the government advice. We discussed the most responsible route and decided to cross the North Sea by ferry from Rotterdam to Hull. Sea turtles are always complicated to send across borders as they are endangered and listed on CITES, even old bones require proof that they existed before the animal was added to the CITES convention (a condition which we satisfy by several thousand years!). Willemien managed to courier them to York just before Brexit officially kicked in on the 1st of January 2021 saving her from more paperwork, phew! Rachel however crossed the sea with extra luggage consisting of ancient Mediterranean fish (none are on the CITES list!), it’s a glamorous life.

Due to the pandemic, acquiring samples has been predictably challenging. Closures to museums and institutions responsible for granting permission to destructively sample these materials, has resulted in delays to this project where only a subset of samples have been analysed to date. Fortunately, bluefin tuna are not listed on CITES which would have complicated matters further. 

Upon arrival into the UK, there were mandatory quarantine periods accompanied by the stress of hoping the courier finds the house and picks up our at home COVID tests, waiting for those results (negative, thankfully!), and then being permitted to arrange starting lab work. Once lab work commenced with regularity there are new considerations to going into the lab versus in non-covid times. For example, having to complete all of your training with colleagues at least 2 metres away and only being permitted a finite number of people in a space at any given time. To orchestrate all of this and ensure necessary precautions were taken, this further involved signing up on booking sheets for time slots in lab spaces, getting tested for covid twice a week, and when space was limited, working in slightly atypical spaces (casting sidelong looks to the zooarchaeology laboratory).

Of course, for one of us (the Englishman), quarantine was a relaxing (and merry) stint at home over the Christmas break.

Whilst we could access lab space and prepare samples for isotopic analysis, the experience was (not shockingly) simply incomparable with pre-pandemic times. Despite this, we still shared jokes in the lab that lightened the mood and made us feel very welcome and positive about the research. Even with so few colleagues around, we were very well supported when teething problems in new protocols/equipment arose and are grateful to everyone in BioArch at the University of York for enabling us to complete our lab work as planned.

Come plague, come ice and snow..samples will be processed,
Adam (left), Rachel (center) and Willemien (right) in front of York minster.

The three of us managed to extract collagen from an impressive number of samples (nearly 500 in total) and we were lucky that the York BioArch crew were so patient with us especially when we were using ALL of the test tubes. There was not much else to do except work, but eventually that played to our advantage when we saw the collagen come out of the freeze-drier looking exactly like cotton-candy – admittedly not as sweet but just as satisfying.

Stay tuned for the results coming to a journal near you!

Contribution of food residues preserved in archaeological ceramics to the understanding of animal husbandry.

Emmanuelle Casanova

Organic Geochemistry Unit, University of Bristol and soon to be a MSCA IF fellow at APPSPE, MNHN, Paris.

Food residues in ancient ceramics

Pottery vessels appeared during the Neolithic and were used extensively to store, transport, cook and process food. During cooking, grease from the food migrates into the vessel walls and survive there through the ages, either complete or in a degraded form. These lipidic residues can be recovered and analysed at the molecular level. Based on the presence of specific molecules, called biomarkers, and their distribution, the source of food that was processed in the vessels is identifiable. Lipid residues from animal products are by far the most common residues recovered in the ceramics but it is also possible to find evidence of beeswax, plants, vegetables and even cereals.

Animal fats survive in the archaeological context in a hydrolysed from with the palmitic and stearic acids as the two major compounds. Measuring the stable carbon isotope values (δ13C) of these individual fatty acids allows to identify whether the fats came from non-ruminant, ruminant animals, and aquatic sources. This method is particularly interesting to highlight the exploitation of the dairy products of domesticated animals. It is, nonetheless, not species-specific and correlation of lipid data with the faunal records allows for further understanding about the source of animal fats cooked and processed in ceramics.

Example of Neolithic pottery from the Linearbankeramik of the Upper-Alsace analysed for its absorbed lipid residues.

The example of Alsatian Neolithic

I studied the region of Alsace for my PhD project, a part of the NeoMilk project. During the Neolithic, two sub-groups of the Linearbandkeramik culture originating from two separate migration waves settled following the modern border of Lower and Upper Alsace. Faunal remains were dominated by domesticated animals (>96%) particularly cattle, then pig and caprines (sheep/goat). The number of identified specimens in an assemblage can be weighted for the meat available per animal (i.e. if a caprine is 1 unit, a pig is 3.3 units and a cow 7.3 units for Neolithic animal) for the data to be comparable to the abundance of lipid sources in the ceramics. A noticeable difference from the faunal remains in the two groups is the availability of pig meat being ~30% in Lower Alsace but ~5% in the Upper Alsace.

A. Map of the Alsace region showing the boundary between the Lower and Upper Alsace and the sites selected for lipid residue analyses. B. Pottery with decorative motifs representative of successive LBK phases of the regional groups in Alsace and relative chronology (Lefranc, 2007).

Ruminant meat and milk were extensively processed in pottery vessels of the Upper Alsace, while non-ruminant products were scarce. On the contrary, in the Lower-Alsace both non-ruminant and ruminant meat dominate the residues in the ceramics, while dairy products were scarce. The results support that the two contemporaneous sub-groups of the Linearbandkeramik exploiting their herds differently with a particular focus on ruminant products, including dairy in the Upper Alsace.

Typical meat weight of the faunal remains for Linearbandkeramik groups of the Lower Alsace and Upper Alsace and results of compound-specific stable carbon isotope analyses on fatty acids extracted from pottery vessels

Direct radiocarbon dating of food residues: what can it tell us?

Radiocarbon dating is a key technique used to resolve the chronology of archaeological sites. I developed during my PhD and postdoc a method for directly dating ceramics from their preserved animal fats as a compound-specific approach. Among other applications, it permits to resolve the antiquity specific food commodities such as ruminant dairy by directly dating the commodity. I have applied this method to the dating of ruminant dairy residues in several regions of Europe and North Africa but also to the dating of equine products in Central Asia.

The method is particularly interesting at sites where evidence for the exploitation of a specific food commodity is scarce or unsupported by other archaeological and archaeozoological evidence. For instance, I applied the method to the radiocarbon dating of dairy residues from ceramics associated with hunter-gatherers of the Lesotho groups of 1st Millennium AD. The presence of dairy residues and their age compatible with the hunter-gatherer groups provided evidence of their access to domesticated animals, a parameter which was debated based on the morphological and ancient DNA analyses of the faunal assemblage.

These studies demonstrate the potential for organic residue analysis to enhance our understanding of past human-animal interactions. My new project, VARGAH, funded as part of the MSCA-IF will start in June hosted by Dr Marjan Maskhour at the AAPSPE, MNHN (Paris, France), uncovering the economy and chronology of early sendentary and mobile pastoralists in Iran.

My PhD was funded by the NeoMilk project (ERC-advanced grant awarded to Prof. Richard Evershed), subsquently followed by a post-doc funded by a ERC proof of concept project (LipDat awarded to Prof. Richard Evershed).

Intra-individual Sequential Carbon and Oxygen Isotope Analyses of Neolithic Livestock for Assessing the Early Husbandry Strategies in the Southern Caucasus

Masato Hirose

Department of Earth and Environmental Sciences at Nagoya University

By sequential sampling and analyzing tooth enamel, we can read the seasonal variations in stable isotope ratios recorded in teeth. Seasonal variations in the stable carbon (δ13C) and oxygen (δ18O) isotopes can be used to infer what the individual consumed, where, and in what season. In other words, this method is suitable for studying the husbandry strategies from livestock remains. However, in the analysis of an isolated tooth, the term of the obtained variation would be short, and difficult to capture the feature of the variation. Analyzing two teeth per individual can provide long-term variation, although this requires the use of well-preserved archaeological materials.

Excavations at Göytepe (Photo: Dr. Seiji Kadowaki)

In our new study, we used this intra-individual sequential isotope analysis with M2 and M3 teeth per individual as far as possible to investigate early husbandry strategies in the southern Caucasus. We analyzed the mandibular tooth enamel of goats, sheep, and cattle (only isolated teeth) from the Neolithic settlements, Göytepe (ca. 5650–5460 cal BC) and Hacı Elamxanlı Tepe (ca. 5950–5800 cal BC) located in western Azerbaijan. As reference samples, we also analyzed modern goat and sheep individuals that are known to have grazed in the vicinity of the sites.

Map of the southern Caucasus, showing the location of Göytepe, Hacı Elamxanlı Tepe, and other main Neolithic sites belonging to the Shomutepe-Shulaveri culture. The locations of the obsidian sources are only those where the use of the obsidian was recognized in Göytepe materials (Nishiaki et al., 2019).

In this region, the first full-fledged Neolithic agro-pastoral economy called the Shomutepe-Shulaveri culture, emerged suddenly around 6000 cal BC. Despite their geographical closeness to each other, this is about 2000 to 3000 years later than the emergence of agro-pastoral practices in the Fertile Crescent. It is important to study the delay in this phenomenon in detail in order to understand the process of diffusion of agro-pastoral economy in human society. Therefore, in this study, we focused on how early agro-pastoral economy was practiced in this region under the peculiar environmental conditions colder in winter, adjacent to mountainous regions, that are uncommon in the Fertile Crescent. Livestock management regarding mobility and migration is a key aspect in understanding agro-pastoral societies. Therefore, we attempted to provide isotopic evidence indicative of early husbandry practices in the southern Caucasus.

Sequential stable carbon and oxygen isotope values of the Neolithic livestock from Göytepe.

The obtained data showed several different patterns that may be explained by different modes of husbandry practices:

  1. Some of the goats and sheep exhibit large amplitudes in δ13C and δ18O variations (see figure above: part a). This was interpreted as a lowland pasturing pattern because the modern goat and sheep showed the same pattern.
  2. A Neolithic goat and three Neolithic cattle samples exhibited relatively small amplitudes and/or inverse cyclical variation patterns (figure b and d). While these patterns may have been caused by multiple factors, such as drinking water and food/fodder, vertical transhumance has also been proposed to result in the similar isotope patterns (e.g., Henton et al., 2010; Tornero et al., 2016). If animals experience seasonal vertical transhumance between lowlands in winter and highlands in summer, it is expected that the δ13C fluctuation range reduces. In addition, the δ13C value of C4 plant feeders is presumed to decrease if they spend summer in highlands where C4 plants are less.
  3. Some individuals illustrated a pattern with a larger amplitude of δ18O seasonal variations but a smaller amplitude of δ13C variations (c). It is likely that some factor reduced the variation of δ13C. We proposed a possibility of the use of fodder (C3 plants) collected in a short term and given to livestock thus dampening the seasonal variation in δ13C.

The factors contributing to the patterns of isotopic variation presented in our study may not be limited to transhumance or the use of fodder but are consistent with such possibilities. To verify these hypotheses, other analytical methods, such as strontium isotope analysis, are required to specify pasturing places in different seasons on the basis of a local and regional isoscape. In any case, at least, these various sequential isotopic patterns suggest that a variety of livestock breeding strategies were already adopted by Neolithic inhabitants in the southern Caucasus. Thus, long-term sequential isotope data from plural teeth per individual would provide us more specific seasonal variation patterns.

This research derives from the Azerbaijani-Japanese Archaeological Mission directed by Prof. Yoshihiro Nishiaki (The University of Tokyo) and Dr. Farhad Guliyev (The National Academy of Science, Azerbaijan). The financial support for this study was provided by the JSPS KAKENHI (No. 17H04534), the MEXT KAKENHI (No. 16H06408 and 20H00026), and The Takanashi Foundation for Historical Science.

A consistent desire for animal proteins in the Bronze Age Xinjiang, China

Minghao Lin

Department of History, Shanghai Jiao Tong University, 200240 Shanghai, China

Location of Xinjiang (China)

The Xinjiang region is located in far northwestern China neighboring central Asia and Mongolian Steppe. Throughout antiquity it has been a nexus for exchange in ideas, technologies, livestock, or even populations. Although most of its land is covered by severe environments such as the Taklamakan Desert, diverse cultures have been recognised since the early Bronze Age. Near Eastern cultivars such as wheat and barley have been excavated and dated to by the beginning of the second millennium BC in this region. In spite of the appearance of wheat and barley at some sites, did the subsistence of ancient Xinjiang populations really rely on these novel C3 cultivars? The degree to which these Near Eastern cultivars contributed to human dietary intake and how these new food types shaped local subsistence strategies remains poorly understood.

The northern slope of the East Tian Shan Mountains in Balikun, Xinjiang (China). (Credit: Minghao Lin)

In this research, part of my Humboldt fellowship project hosted by Prof Cheryl Makarewicz at Kiel University, we conducted a regional scale meta-analyses of carbon and nitrogen isotope values measured from humans and animals including cattle, sheep/goats, and deer from sites dating from c. 2000 BC to AD 1000 within the Xinjiang region to examine the degree to which novel cultivars and animal proteins were incorporated into local subsistence system from the perspective of isotopic expression. We notice a narrow gap (1.7-2.7‰) in δ13C values but a wide space (4.5-6.5‰) in δ15N between humans and bovid samples. Meanwhile, we also record a consistent expression of high nitrogen isotope values visible in humans across time, indicating a persistent preference of the Xinjiang people for animal resources (e.g. meat, dairy proteins). This suggests novel cultivars of wheat and barley were not rapidly adopted in Xinjiang highlighting the food dispersal and acceptance across Eurasia during prehistory was a complex process.

Results of meta-analysis of stable carbon and nitrogen isotopic results from Xinjiang (China)
(Credit: Minghao Lin).

Current Methods and Best Practices in Strontium Isotope Mapping

Emily Holt, Jane A. Evans, and Richard Madgwick

Drawing on variations in bioavailable strontium in different environments to provenance biological materials has become increasingly common since its first application in archaeology almost four decades ago, and it is frequently applied to zooarchaeological materials. Provenancing biological materials, including faunal remains, using strontium isotope ratios generally requires a map of bioavailable strontium, commonly known as an isoscape, to compare results with. However, both producing the isoscape and using it to interpret results present methodological challenges that researchers must carefully consider.

To help researchers understand the complexities of strontium analysis, we reviewed current research to produce a critical synthesis and recommend best practices. We addressed sampling the archives needed to build an isoscape, applying the different mapping and modeling methods currently available, and interpreting the results of archaeological analyses against isoscapes. Our critical review is freely available to read and download from Earth-Science Reviews until May 8, 2021


Current research indicates that, while many archives can be analyzed to produce isoscapes, modern plant materials usually provide the best approximation of bioavailable strontium. Modern plants can be used alone or combined with other archives if applying a machine learning approach to mapping. In areas where erosion has significantly shifted the location of surface sediments, modern plants may not provide an appropriate archive for building an isoscape that is applicable to archaeological materials. In these areas, alternative archives such as archaeological rodent remains may be preferable. Archaeologists should also be aware that contemporary soil treatments like fertilizers may affect the bioavailable strontium in soils. Areas used for agriculture should be avoided when sampling.

Strontium pathways (adapted from Bataille et al. 2020, Fig. 1)

It is essential to collect appropriate metadata when sampling. Collecting metadata improves the legacy benefits of strontium data by making them more broadly applicable. These metadata and the results of the analyses should be archived in one of several online databases to maximize their usefulness.

Mapping and modeling

We found that domain mapping currently produces the most accurate, most interpretable isoscapes for most research questions. However, machine learning approaches are also powerful and promise to provide more accurate and geographically wide-ranging isoscapes over time. Machine learning is computationally intensive and may not currently be an option for many researchers, but will be more widely available in the future.

Strontium isoscapes that are both appropriate and sufficiently high resolution to answer specific research questions do not exist for most parts of the world. Researchers intending to incorporate strontium analysis into their research designs should expect to conduct primary sampling and analysis to create appropriate isoscapes or refine existing ones, which should themselves not be utilized uncritically.


Strontium isotope analysis is currently the best developed and understood provenance tool. However, because isotope data can only exclude options of possible origin, the development of multi-isotope methods provides the route to a more powerful future approach. Using strontium isotope analysis for provenancing is most successful when combined with other isotopes and/or trace elements as part of a likelihood approach.

Future directions

In the future, we expect that increasing amounts of primary data and the increasing application of machine learning approaches to mapping will mean that strontium analysis continues to improve as a method of provenancing.

This research was apart of the ZANBA – Zooarchaeology of the Nuragic Bronze Age, a Marie Sklodowska Curie fellowship awarded to Emily Holt.